November 13, 2025 Webinar
Venturing Beyond Genomics: Integrating Chromatin, Transcript, and Protein Layers in Single Cells
Avi Srivastava, PhD
Abstract:
Recent advances in single-cell genomics have revealed striking cellular heterogeneity in health and disease, yet most approaches remain confined to a single modality. My lab develops experimental and computational frameworks for multimodal single-cell analysis - integrating chromatin state, RNA expression, and protein phenotype within unified statistical models. I will describe two methods that extend the single-cell toolkit: scCUT&Tag-pro, which co-assays histone modifications and surface proteins, and scChromHMM, which models combinatorial chromatin states across individual cells using probabilistic inference. Together, these tools enable quantitative modeling of gene-regulatory logic at single-cell resolution, opening new opportunities to study chromatin-state dynamics and isoform-level regulation in cancer and immune systems.
Short Bio:
Avi Srivastava, Ph.D., is an Assistant Professor at The Wistar Institute, where his group develops quantitative frameworks and technologies for multimodal single-cell genomics. His lab combines statistical modeling, algorithm design, and scalable sequencing to connect chromatin, transcript, and protein information at single-cell resolution. Dr. Srivastava co-developed algorithms in Seurat and Signac for integrative analysis and contributed to the Salmon–Alevin suite for transcript quantification. His research focuses on building reproducible, computationally efficient, and accessible methods to study gene-regulatory networks in cancer and immune systems.
Hope to see you at the webinar!